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ASMT Rabbit pAb (A6529)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ASMT Rabbit pAb (A6529)

Western blot analysis of various lysates, using ASMT Rabbit pAb (A6529) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ASMT Rabbit pAb (A6529)

Immunohistochemistry analysis of paraffin-embedded Rat heart using ASMT Rabbit pAb (A6529) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ASMT Rabbit pAb (A6529)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using ASMT Rabbit pAb (A6529) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameASMT Rabbit pAb
Catalog No.A6529
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human ASMT (NP_001164510.1).
SequenceMGSSEDQAYRLLNDYANGFMVSQVLFAACELGVFDLLAEAPGPLDVAAVAAGVRASAHGTELLLDICVSLKLLKVETRGGKAFYRNTELSSDYLTTVSPTSQCSMLKYMGRTSYRCWGHLADAVREGRNQYLETFGVPAEELFTAIYRSEGERLQFMQALQEVWSVNGRSVLTAFDLSVFPLMCDLGGDFFKDPLPEADLYILARVLHDWADGKCSHLLERIYHTCKPGGGILVIESLLDEDRRGPLLTQLYSLNMLVQTEGQERTPTHYHMLLSSAGFRDFQFKKTGAIYDAILARK
Gene ID
Swiss Prot
SynonymsASMTY; HIOMT; HIOMTY; ASMT
Calculated MW38kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293F, Y79
Cellular locationcytosol.

Documents

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ABclonal:Western blot - ASMT Rabbit pAb (A6529)}

Western blot - ASMT Rabbit pAb (A6529)

Western blot analysis of various lysates, using ASMT Rabbit pAb (A6529) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ASMT Rabbit pAb (A6529)}

Immunohistochemistry - ASMT Rabbit pAb (A6529)

Immunohistochemistry analysis of paraffin-embedded Rat heart using ASMT Rabbit pAb (A6529) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ASMT Rabbit pAb (A6529)}

Immunohistochemistry - ASMT Rabbit pAb (A6529)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using ASMT Rabbit pAb (A6529) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)