제품 > 항체 > 단일클론항체(mAb)

ASUN/INTS13 Rabbit mAb (A22792)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ASUN/INTS13 Rabbit mAb (A22792)

Western blot analysis of various lysates, using ASUN/INTS13 Rabbit mAb (A22792) at 1:70000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameASUN/INTS13 Rabbit mAb
Catalog No.A22792
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55758
Involved in regulation of mitotic cell cycle. Acts upstream of or within centrosome localization; mitotic spindle organization; and protein localization to nuclear envelope. Located in cytoplasm and nuclear body.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human ASUN/INTS13. (NP_060634.2).
SequenceQKCELVLIHTYPVGEDSLVSDRSKKELSPVLTSEVHSVRAGRHLATKLNILVQQHFDLASTTITNIPMKEEQHANTSANYDVELLHHKDAHVDFLKSGDSH
Gene ID
Swiss Prot
SynonymsASUN; GCT1; NET48; Mat89Bb; SPATA30; C12orf11; ASUN/INTS13
Calculated MW80kDa
Observed MW80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:80000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU266, 293F, HeLa
Cellular location

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Lot number

    ABclonal:Western blot - ASUN/INTS13 Rabbit mAb (A22792)}

    Western blot - ASUN/INTS13 Rabbit mAb (A22792)

    Western blot analysis of various lysates, using ASUN/INTS13 Rabbit mAb (A22792) at 1:70000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.