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ATP1A4 Rabbit mAb (A23196)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP1A4 Rabbit mAb (A23196)

Western blot analysis of lysates from Mouse brain, using ATP1A4 Rabbit mAb (A23196) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - ATP1A4 Rabbit mAb (A23196)

Western blot analysis of various lysates, using ATP1A4 Rabbit mAb (A23196) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ATP1A4 Rabbit mAb (A23196)

Immunohistochemistry analysis of ATP1A4 in paraffin-embedded rat testis tissue using ATP1A4 Rabbit mAb (A23196) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATP1A4 Rabbit mAb (A23196)

Immunohistochemistry analysis of ATP1A4 in paraffin-embedded mouse testis tissue using ATP1A4 Rabbit mAb (A23196) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ATP1A4 Rabbit mAb (A23196)

Immunofluorescence analysis of paraffin-embedded mouse testis using ATP1A4 Rabbit mAb (A23196) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameATP1A4 Rabbit mAb
Catalog No.A23196
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59773
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 401-530 of human ATP1A4 (NP_653300.2)
SequenceFDMTVYEADTTEEQTGKTFTKSSDTWFMLARIAGLCNRADFKANQEILPIAKRATTGDASESALLKFIEQSYSSVAEMREKNPKVAEIPFNSTNKYQMSIHLREDSSQTHVLMMKGAPERILEFCSTFLL
Gene ID
Swiss Prot
SynonymsATP1A1; ATP1AL2; ATP1A4
Calculated MW19KDa/114KDa
Observed MW113kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesbewo, MCF7, Mouse brain
Cellular locationCell membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ATP1A4 Rabbit mAb (A23196)}

    Western blot - ATP1A4 Rabbit mAb (A23196)

    Western blot analysis of lysates from Mouse brain, using ATP1A4 Rabbit mAb (A23196) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - ATP1A4 Rabbit mAb (A23196)}

    Western blot - ATP1A4 Rabbit mAb (A23196)

    Western blot analysis of various lysates, using ATP1A4 Rabbit mAb (A23196) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - ATP1A4 Rabbit mAb (A23196)}

    Immunohistochemistry - ATP1A4 Rabbit mAb (A23196)

    Immunohistochemistry analysis of ATP1A4 in paraffin-embedded rat testis tissue using ATP1A4 Rabbit mAb (A23196) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ATP1A4 Rabbit mAb (A23196)}

    Immunohistochemistry - ATP1A4 Rabbit mAb (A23196)

    Immunohistochemistry analysis of ATP1A4 in paraffin-embedded mouse testis tissue using ATP1A4 Rabbit mAb (A23196) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - ATP1A4 Rabbit mAb (A23196)}

    Immunofluorescence - ATP1A4 Rabbit mAb (A23196)

    Immunofluorescence analysis of paraffin-embedded mouse testis using ATP1A4 Rabbit mAb (A23196) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)