제품 > 항체 > 다중클론항체(pAb)

ATP23 Rabbit pAb (A12885)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP23 Rabbit pAb (A12885)

Western blot analysis of various lysates using ATP23 Rabbit pAb (A12885) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ATP23 Rabbit pAb (A12885)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ATP23 Rabbit pAb (A12885) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATP23 Rabbit pAb (A12885)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using ATP23 Rabbit pAb (A12885) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ATP23 Rabbit pAb (A12885)

Immunofluorescence analysis of MCF7 cells using ATP23 Rabbit pAb (A12885) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameATP23 Rabbit pAb
Catalog No.A12885
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is amplified in glioblastomas and interacts with the DNA binding subunit of DNA-dependent protein kinase. This kinase is involved in double-strand break repair (DSB), and higher expression of the encoded protein increases the efficiency of DSB. In addition, comparison to orthologous proteins strongly suggests that this protein is a metalloprotease important in the biosynthesis of mitochondrial ATPase. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human ATP23 (NP_150592.1).
SequenceMAGAPDERRRGPAAGEQLQQQHVSCQVFPERLAQGNPQQGFFSSFFTSNQKCQLRLLKTLETNPYVKLLLDAMKHSGCAVNKDRHFSCEDCNGNVSGGFDASTSQIVLCQNNIHNQAHMNRVVTHELIHAFDHCRAHVDWFTNIRHLACSEVRAANLSGDCSLVNEIFRLHFGLKQHHQTCVRDRATLSILAVRNISKEVAKKAVDEVFESCFNDHEPFGRIPHNKTYARYAHRDFENRDRYYSNI
Gene ID
Swiss Prot
SynonymsKUB3; XRCC6BP1; ATP23
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:3000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationcytosol, extrinsic component of mitochondrial inner membrane, plasma membrane.

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ABclonal:Western blot - ATP23 Rabbit pAb (A12885)}

Western blot - ATP23 Rabbit pAb (A12885)

Western blot analysis of various lysates using ATP23 Rabbit pAb (A12885) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ATP23 Rabbit pAb (A12885)}

Immunohistochemistry - ATP23 Rabbit pAb (A12885)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ATP23 Rabbit pAb (A12885) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ATP23 Rabbit pAb (A12885)}

Immunohistochemistry - ATP23 Rabbit pAb (A12885)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using ATP23 Rabbit pAb (A12885) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - ATP23 Rabbit pAb (A12885)}

Immunofluorescence - ATP23 Rabbit pAb (A12885)

Immunofluorescence analysis of MCF7 cells using ATP23 Rabbit pAb (A12885) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)