제품 > 항체 > 다중클론항체(pAb)

ATP5C1 Rabbit pAb (A15257)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP5C1 Rabbit pAb (A15257)

Western blot analysis of various lysates, using ATP5C1 Rabbit pAb (A15257) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)

Immunohistochemistry analysis of paraffin-embedded Human placenta using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - ATP5C1 Rabbit pAb (A15257)

Immunofluorescence analysis of C6 cells using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATP5C1 Rabbit pAb (A15257)

Immunofluorescence analysis of L929 cells using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATP5C1 Rabbit pAb (A15257)

Immunofluorescence analysis of U-2 OS cells using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameATP5C1 Rabbit pAb
Catalog No.A15257
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1).
SequenceATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARIYGLGSLALYEKADIKGPEDKKKHLLIGVSSDRGLCGAIHSSIAKQMKSEVATLTAAGKEVMLVGIGDKIRGILYRTHSDQFLVAFKEVGRKPPTFGDASVIALELLNSGYEFDEGSIIFNKFRSVISYKTEEKPIFSLNTVASADSMSIYDDIDADVLQNYQEYNLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD
Gene ID
Swiss Prot
SynonymsATP5C; ATP5C1; ATP5CL1
Calculated MW33kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart, Mouse brain
Cellular locationMitochondrion, Mitochondrion inner membrane, Peripheral membrane protein.

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ABclonal:Western blot - ATP5C1 Rabbit pAb (A15257)}

Western blot - ATP5C1 Rabbit pAb (A15257)

Western blot analysis of various lysates, using ATP5C1 Rabbit pAb (A15257) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)}

Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)}

Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)}

Immunohistochemistry - ATP5C1 Rabbit pAb (A15257)

Immunohistochemistry analysis of paraffin-embedded Human placenta using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - ATP5C1 Rabbit pAb (A15257)}

Immunofluorescence - ATP5C1 Rabbit pAb (A15257)

Immunofluorescence analysis of C6 cells using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATP5C1 Rabbit pAb (A15257)}

Immunofluorescence - ATP5C1 Rabbit pAb (A15257)

Immunofluorescence analysis of L929 cells using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATP5C1 Rabbit pAb (A15257)}

Immunofluorescence - ATP5C1 Rabbit pAb (A15257)

Immunofluorescence analysis of U-2 OS cells using ATP5C1 Rabbit pAb (A15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)