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ATP5G1 Rabbit mAb (A20961)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP5G1 Rabbit mAb (A20961)

Western blot analysis of extracts of various cell lines, using ATP5G1 antibody (A20961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ATP5G1 Rabbit mAb (A20961)

Immunofluorescence analysis of mouse heart using ATP5G1 antibody (A20961) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameATP5G1 Rabbit mAb
Catalog No.A20961
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2949
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP5G1 (P05496).
SequenceMQTAGALFISPALIRCCTRGLIRPVSASFLNSPVNSSKQPSYSNFPLQVARREFQTSVVSRDIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARN
Gene ID
Swiss Prot
SynonymsATP5A; ATP5G; ATP5G1
Calculated MW14kDa
Observed MW8kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHL-60, Mouse heart, Mouse small intestine, Mouse liver, Rat heart, Rat brain
Cellular locationmitochondrial inner membrane, mitochondrial proton-transporting ATP synthase complex, mitochondrial proton-transporting ATP synthase complex, coupling factor F(o), mitochondrion

Documents

Certificate of Compliance

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ABclonal:Western blot - ATP5G1 Rabbit mAb (A20961)}

Western blot - ATP5G1 Rabbit mAb (A20961)

Western blot analysis of extracts of various cell lines, using ATP5G1 antibody (A20961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - ATP5G1 Rabbit mAb (A20961)}

Immunofluorescence - ATP5G1 Rabbit mAb (A20961)

Immunofluorescence analysis of mouse heart using ATP5G1 antibody (A20961) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)