제품 > 항체 > 다중클론항체(pAb)

ATP5L Rabbit pAb (A9178)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP5L Rabbit pAb (A9178)

Western blot analysis of various lysates using ATP5L Rabbit pAb (A9178) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of HeLa cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of MCF7 cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of NIH/3T3 cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of PC-12 cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameATP5L Rabbit pAb
Catalog No.A9178
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4).
SequenceMAQFVRNLVEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVKEAVLNGLVATEVLMWFYVGEIIGKRGIIGYDV
Gene ID
Swiss Prot
SynonymsATP5L; ATP5JG
Calculated MW11kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, A-549, HepG2, HeLa, Mouse brain, Mouse liver, Rat brain, Rat kidney
Cellular locationMitochondrion, Mitochondrion inner membrane

Documents

Certificate of Compliance

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ABclonal:Western blot - ATP5L Rabbit pAb (A9178)}

Western blot - ATP5L Rabbit pAb (A9178)

Western blot analysis of various lysates using ATP5L Rabbit pAb (A9178) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)}

Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of HeLa cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)}

Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of MCF7 cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)}

Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of NIH/3T3 cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATP5L Rabbit pAb (A9178)}

Immunofluorescence - ATP5L Rabbit pAb (A9178)

Immunofluorescence analysis of PC-12 cells using ATP5L Rabbit pAb(A9178) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)