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ATP6 Rabbit pAb (A8193)

Publications (12) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ATP6 Rabbit pAb (A8193)

Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit pAb (A8193) at 1:2000；1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - ATP6 Rabbit pAb (A8193)

Immunofluorescence analysis of U2OS cells using ATP6 Rabbit pAb (A8193) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameATP6 Rabbit pAb
Catalog No.A8193
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to contribute to proton-transporting ATP synthase activity, rotational mechanism. Predicted to be involved in mitochondrial ATP synthesis coupled proton transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; heart; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; NARP syndrome; Parkinson's disease; multiple sclerosis; and systemic lupus erythematosus. Orthologous to human MT-ATP6 (mitochondrially encoded ATP synthase membrane subunit 6).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 127-226 of mouse ATP6 (NP_904333.1).
SequenceHFLPQGTPISLIPMLIIIETISLFIQPMALAVRLTANITAGHLLMHLIGGATLVLMNISPPTATITFIILLLLTILEFAVALIQAYVFTLLVSLYLHDNT
Gene ID
Swiss Prot
SynonymsMT-ATP6; MTATP6; ATPASE6
Calculated MW25kDa
Observed MW20kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein.
Customer validation

WB(Sus scrofa, Homo sapiens, Mus musculus, Rattus norvegicus, Other)

IF(Mus musculus)

IHC(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - ATP6 Rabbit pAb (A8193)}

    Western blot - ATP6 Rabbit pAb (A8193)

    Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit pAb (A8193) at 1:2000；1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunofluorescence - ATP6 Rabbit pAb (A8193)}

    Immunofluorescence - ATP6 Rabbit pAb (A8193)

    Immunofluorescence analysis of U2OS cells using ATP6 Rabbit pAb (A8193) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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