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ATP6 Rabbit pAb (A8193)

Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ATP6 Rabbit pAb (A8193)

Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit pAb (A8193) at 1:2000;1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - ATP6 Rabbit pAb (A8193)

Immunofluorescence analysis of U2OS cells using ATP6 Rabbit pAb (A8193) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameATP6 Rabbit pAb
Catalog No.A8193
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to contribute to proton-transporting ATP synthase activity, rotational mechanism. Predicted to be involved in mitochondrial ATP synthesis coupled proton transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; heart; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; NARP syndrome; Parkinson's disease; multiple sclerosis; and systemic lupus erythematosus. Orthologous to human MT-ATP6 (mitochondrially encoded ATP synthase membrane subunit 6).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 127-226 of mouse ATP6 (NP_904333.1).
SequenceHFLPQGTPISLIPMLIIIETISLFIQPMALAVRLTANITAGHLLMHLIGGATLVLMNISPPTATITFIILLLLTILEFAVALIQAYVFTLLVSLYLHDNT
Gene ID
Swiss Prot
SynonymsMT-ATP6; MTATP6; ATPASE6
Calculated MW25kDa
Observed MW20kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein.
Customer validation

WB(Sus scrofa, Homo sapiens, Mus musculus, Rattus norvegicus)

Documents

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    ABclonal:Western blot - ATP6 Rabbit pAb (A8193)}

    Western blot - ATP6 Rabbit pAb (A8193)

    Western blot analysis of lysates from HepG2 cells, using ATP6 Rabbit pAb (A8193) at 1:2000;1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunofluorescence - ATP6 Rabbit pAb (A8193)}

    Immunofluorescence - ATP6 Rabbit pAb (A8193)

    Immunofluorescence analysis of U2OS cells using ATP6 Rabbit pAb (A8193) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8193? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)