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ATP6V0C Rabbit mAb (A23757)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ATP6V0C Rabbit mAb (A23757)

Western blot analysis of various lysates, using ATP6V0C Rabbit mAb (A23757) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameATP6V0C Rabbit mAb
Catalog No.A23757
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61235
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP6V0C. (NP_001685.1).
SequenceMSESKSGPEYASFFAVMGASAAMVFSALGAAYGTAKSGTGIAAMSVMRPEQIMKSIIPVVMAGIIAIYGLVVAVLIANSLNDDISLYKSFLQLGAGLSVG
Gene ID
Swiss Prot
SynonymsATP6V0C; ATP6C; ATP6L; ATPL; VATL; VPPC; Vma3; ATPase H+ transporting V0 subunit c
Calculated MW15kDa
Observed MW16kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, 293F
Cellular locationMulti-pass membrane protein, Vacuole membrane
Customer validation

WB(Mus musculus)

Documents

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    ABclonal:Western blot - ATP6V0C Rabbit mAb (A23757)}

    Western blot - ATP6V0C Rabbit mAb (A23757)

    Western blot analysis of various lysates, using ATP6V0C Rabbit mAb (A23757) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A23757? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)