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ATP6V0C Rabbit pAb (A16350)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP6V0C Rabbit pAb (A16350)

Western blot analysis of various lysates using ATP6V0C Rabbit pAb (A16350) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - ATP6V0C Rabbit pAb (A16350)

Immunofluorescence analysis of MCF7 cells using ATP6V0C Rabbit pAb (A16350) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameATP6V0C Rabbit pAb
Catalog No.A16350
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATP6V0C (NP_001185498.1).
SequenceMSESKSGPEYASFFAVMGASAAMVFSALGAAYGTAKSGTGIAAMSVMRPEQIMKSIIPVVMAGIIAIYGLVVAVLIANSLNDDISLYKSFLQLGAGLSVG
Gene ID
Swiss Prot
SynonymsATPL; VATL; VPPC; Vma3; ATP6C; ATP6L; ATP6V0C
Calculated MW16kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Rat brain
Cellular locationMulti-pass membrane protein, Vacuole membrane.
Customer validation

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

Co-IP(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - ATP6V0C Rabbit pAb (A16350)}

    Western blot - ATP6V0C Rabbit pAb (A16350)

    Western blot analysis of various lysates using ATP6V0C Rabbit pAb (A16350) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - ATP6V0C Rabbit pAb (A16350)}

    Immunofluorescence - ATP6V0C Rabbit pAb (A16350)

    Immunofluorescence analysis of MCF7 cells using ATP6V0C Rabbit pAb (A16350) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16350? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)