제품 > 항체 > 다중클론항체(pAb)

ATP6V1G2 Rabbit pAb (A20163)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP6V1G2 Rabbit pAb (A20163)

Western blot analysis of various lysates using ATP6V1G2 Rabbit pAb (A20163) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - ATP6V1G2 Rabbit pAb (A20163)

Immunohistochemistry analysis of paraffin-embedded rat brain using ATP6V1G2 Rabbit pAb (A20163) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ATP6V1G2 Rabbit pAb (A20163)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using ATP6V1G2 Rabbit pAb (A20163) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameATP6V1G2 Rabbit pAb
Catalog No.A20163
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-100 of human ATP6V1G2 (NP_569730.1).
SequenceAQMEVEQYRREREHEFQSKQQAAMGSQGNLSAEVEQATRRQVQGMQSSQQRNRERVLAQLL
Gene ID
Swiss Prot
SynonymsNG38; ATP6G; VMA10; ATP6G2; ATP6V1G2
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, Mouse brain, Rat brain, Mouse eye
Cellular locationcytosol, extrinsic component of synaptic vesicle membrane

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ATP6V1G2 Rabbit pAb (A20163)}

    Western blot - ATP6V1G2 Rabbit pAb (A20163)

    Western blot analysis of various lysates using ATP6V1G2 Rabbit pAb (A20163) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - ATP6V1G2 Rabbit pAb (A20163)}

    Immunohistochemistry - ATP6V1G2 Rabbit pAb (A20163)

    Immunohistochemistry analysis of paraffin-embedded rat brain using ATP6V1G2 Rabbit pAb (A20163) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ATP6V1G2 Rabbit pAb (A20163)}

    Immunohistochemistry - ATP6V1G2 Rabbit pAb (A20163)

    Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using ATP6V1G2 Rabbit pAb (A20163) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)