제품 > 항체 > 다중클론항체(pAb)

ATP7A Rabbit pAb (A8399)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - ATP7A Rabbit pAb (A8399)

Western blot analysis of lysates from Rat lung using ATP7A Rabbit pAb(A8399) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:60s.

Overview

Product nameATP7A Rabbit pAb
Catalog No.A8399
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human ATP7A (NP_000043.4).
SequenceMDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMGFDAVIHNPD
Gene ID
Swiss Prot
SynonymsMK; MNK; DSMAX; SMAX3; ATP7A
Calculated MW163kDa
Observed MW163-200kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat lung
Cellular locationCell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Multi-pass membrane protein, Multi-pass membrane protein, cytosol, trans-Golgi network membrane
Customer validation

WB(Danio rerio)

Documents

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    ABclonal:Western blot - ATP7A Rabbit pAb (A8399)}

    Western blot - ATP7A Rabbit pAb (A8399)

    Western blot analysis of lysates from Rat lung using ATP7A Rabbit pAb(A8399) at 1:1000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime:60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8399? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)