제품 > 항체 > 단일클론항체(mAb)

AZI2 Rabbit mAb (A21159)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AZI2 Rabbit mAb (A21159)

Western blot analysis of extracts of various cell lines, using AZI2 antibody (A21159) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameAZI2 Rabbit mAb
Catalog No.A21159
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2987
AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 [PubMed 14560022]).
ImmunogenRecombinant protein containing a sequence corresponding to amino acids 282-387 of human AZI2 (NP_071906.1).
SequenceYTRHPPLLPNGKALCHTTSSPLPGDVKVLSEKAILQSWTDNERSIPNDGTCFQEHSSYGRNSLEDNSWVFPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQN
Gene ID
Swiss Prot
SynonymsAZ2; NAP1; TILP
Calculated MW45kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHep G2, SH-SY5Y, Mouse testis, Rat testis
Cellular locationcytoplasm

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ABclonal:Western blot - AZI2 Rabbit mAb (A21159)}

Western blot - AZI2 Rabbit mAb (A21159)

Western blot analysis of extracts of various cell lines, using AZI2 antibody (A21159) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)