제품 > 항체 > 다중클론항체(pAb)

Aim2 Rabbit pAb (A22874)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Rat

Western blot analysis of Rat spleen, using Aim2 antibody (A22874) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Aim2 Rabbit pAb
Catalog No.	A22874
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables double-stranded DNA binding activity and identical protein binding activity. Involved in positive regulation of macromolecule metabolic process; pyroptosis; and regulation of defense response. Acts upstream of or within cellular response to interferon-beta. Located in cytoplasm. Part of AIM2 inflammasome complex. Orthologous to human AIM2 (absent in melanoma 2).

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 50-150 of mouse Aim2 (NP_001013801.2).
Sequence	DHLIQSAGAASAVTKAINIFQKLNYMHIANALEEKKKEAERKLMTNTKKRGTQKVENRSQAENCSAASATRSDNDFKEQAATEVCPQAKPQKKQMVAEQEA
Gene ID
Swiss Prot
Synonyms	Gm1313; Ifi210
Calculated MW	40kDa
Observed MW	39kDa

Applications

Reactivity	Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Rat spleen
Cellular location	Cytoplasm, Nucleus