제품 > 항체 > 단일클론항체(mAb)

Anti-TrkB Rabbit mAb (A19832)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of various lysates using Anti-TrkB Rabbit mAb (A19832) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product name	Anti-TrkB Rabbit mAb
Catalog No.	A19832
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2358

Background

Enables brain-derived neurotrophic factor binding activity and brain-derived neurotrophic factor-activated receptor activity. Involved in several processes, including brain-derived neurotrophic factor receptor signaling pathway; nervous system development; and trans-synaptic signaling, modulating synaptic transmission. Acts upstream of or within several processes, including neurogenesis; neuronal action potential propagation; and positive regulation of peptidyl-serine phosphorylation. Located in several cellular components, including early endosome; postsynaptic density; and postsynaptic membrane. Is expressed in several structures, including alimentary system; cardiovascular system; future brain; genitourinary system; and nervous system. Human ortholog(s) of this gene implicated in Alzheimer's disease; autistic disorder; developmental and epileptic encephalopathy 58; major depressive disorder; and morbid obesity. Orthologous to human NTRK2 (neurotrophic receptor tyrosine kinase 2).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 120-430 of mouse TrkB (P15209).
Sequence	INFTRNKLTSLSRRHFRHLDLSDLILTGNPFTCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNMPLANLQIPNCGLPSARLAAPNLTVEEGKSVTLSCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKSNEIPSTDVADQSNREHL
Gene ID
Swiss Prot
Synonyms	Tkrb; trkB; trk-B; GP145-TrkB/GP95-TrkB; Anti-TrkB
Calculated MW	92kDa
Observed MW	90kDa/140kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse spinal cord, Mouse brain, Rat brain
Cellular location	axon, axon terminus, cytoplasm, cytosol, dendrite, dendritic spine, early endosome, endosome, excitatory synapse, glutamatergic synapse, growth cone, perikaryon, perinuclear region of cytoplasm, plasma membrane, postsynapse, postsynaptic density, presynaptic active zone, rough endoplasmic reticulum, terminal bouton
Customer validation	WB(Rattus norvegicus)