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Arp3 Rabbit pAb (A1064)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Arp3 Rabbit pAb (A1064)

Western blot analysis of extracts of various cell lines, using Arp3 antibody (A1064) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

ABclonal:Immunofluorescence - Arp3 Rabbit pAb (A1064)

Immunofluorescence analysis of HeLa cells using Arp3 antibody (A1064). Blue: DAPI for nuclear staining.

Overview

Product nameArp3 Rabbit pAb
Catalog No.A1064
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-418 of human Arp3 (NP_005712.1).
SequenceMAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS
Gene ID
Swiss Prot
SynonymsARP3
Calculated MW47kDa
Observed MW47kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesK-562, HL-60, HeLa, Jurkat, COS-1, COS-7, Mouse spleen
Cellular locationCell projection, Cytoplasm, cytoskeleton
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Arp3 Rabbit pAb (A1064)}

Western blot - Arp3 Rabbit pAb (A1064)

Western blot analysis of extracts of various cell lines, using Arp3 antibody (A1064) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Immunofluorescence - Arp3 Rabbit pAb (A1064)}

Immunofluorescence - Arp3 Rabbit pAb (A1064)

Immunofluorescence analysis of HeLa cells using Arp3 antibody (A1064). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1064? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)