제품 > 항체 > 다중클론항체(pAb)

B3GNT3 Rabbit pAb (A17611)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - B3GNT3 Rabbit pAb (A17611)

Western blot analysis of various lysates using B3GNT3 Rabbit pAb (A17611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - B3GNT3 Rabbit pAb (A17611)

Immunohistochemistry analysis of paraffin-embedded Human liver using B3GNT3 Rabbit pAb (A17611) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - B3GNT3 Rabbit pAb (A17611)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using B3GNT3 Rabbit pAb (A17611) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameB3GNT3 Rabbit pAb
Catalog No.A17611
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the beta-1, 3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human B3GNT3 (NP_055071.2).
SequenceYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQ
Gene ID
Swiss Prot
SynonymsTMEM3; B3GN-T3; B3GNT-3; HP10328; B3GAL-T8; beta3Gn-T3; B3GNT3
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSGC-7901, 293T, Mouse stomach
Cellular locationplasma membrane
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - B3GNT3 Rabbit pAb (A17611)}

Western blot - B3GNT3 Rabbit pAb (A17611)

Western blot analysis of various lysates using B3GNT3 Rabbit pAb (A17611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - B3GNT3 Rabbit pAb (A17611)}

Immunohistochemistry - B3GNT3 Rabbit pAb (A17611)

Immunohistochemistry analysis of paraffin-embedded Human liver using B3GNT3 Rabbit pAb (A17611) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - B3GNT3 Rabbit pAb (A17611)}

Immunohistochemistry - B3GNT3 Rabbit pAb (A17611)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using B3GNT3 Rabbit pAb (A17611) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A17611? Please let us know so that we can cite the reference in this datasheet.