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BAF250 Rabbit pAb (A16648)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BAF250 Rabbit pAb (A16648)

Western blot analysis of extracts of various cell lines, using BAF250 antibody (A16648) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product nameBAF250 Rabbit pAb
Catalog No.A16648
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ATP-dependent chromatin remodeler activity. Contributes to nucleosome binding activity. Acts upstream of or within several processes, including animal organ development; embryonic morphogenesis; and toxin transport. Located in chromatin. Part of SWI/SNF complex; nBAF complex; and npBAF complex. Is expressed in several structures, including alimentary system; brain; early conceptus; genitourinary system; and sensory organ. Used to study Coffin-Siris syndrome. Human ortholog(s) of this gene implicated in Coffin-Siris syndrome 2; breast cancer; carcinoma (multiple); gastrointestinal system cancer (multiple); and neuroblastoma. Orthologous to human ARID1A (AT-rich interaction domain 1A).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1300-1400 of mouse BAF250 (NP_001074288.1).
SequenceNMGTGAPQPNLMPSTPDSGMYSPSRYPPQQQQQQQQQHDSYGNQFSTQGTPSSSPFPSQQTTMYQQQQQNYKRPMDGTYGPPAKRHEGEMYSVPYSAGQGQ
Gene ID
Swiss Prot
SynonymsOsa1; BAF250; BAF250a; Smarcf1; 1110030E03Rik
Calculated MW242kDa
Observed MW260kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, Mouse testis
Cellular locationNucleus
Customer validation

WB(Mus musculus)

    ABclonal:Western blot - BAF250 Rabbit pAb (A16648)}

    Western blot - BAF250 Rabbit pAb (A16648)

    Western blot analysis of extracts of various cell lines, using BAF250 antibody (A16648) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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