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BAG3 Rabbit mAb (A4720)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - BAG3 Rabbit mAb (A4720)

Western blot analysis of various lysates using BAG3 Rabbit mAb (A4720) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - BAG3 Rabbit mAb (A4720)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using BAG3 Rabbit mAb (A4720) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameBAG3 Rabbit mAb
Catalog No.A4720
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1104
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human BAG3 (O95817).
SequenceNDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQ
Gene ID
Swiss Prot
SynonymsBIS; MFM6; BAG-3; CAIR-1; BAG3
Calculated MW62kDa
Observed MW74kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, K-562, U-251MG
Cellular locationCytoplasm, Cytosol, Nucleus, Z disc

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ABclonal:Western blot - BAG3 Rabbit mAb (A4720)}

Western blot - BAG3 Rabbit mAb (A4720)

Western blot analysis of various lysates using BAG3 Rabbit mAb (A4720) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BAG3 Rabbit mAb (A4720)}

Immunohistochemistry - BAG3 Rabbit mAb (A4720)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using BAG3 Rabbit mAb (A4720) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)