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BAG3 Rabbit pAb (A14826)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BAG3 Rabbit pAb (A14826)

Western blot analysis of various lysates using BAG3 Rabbit pAb (A14826) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - BAG3 Rabbit pAb (A14826)

Immunohistochemistry analysis of paraffin-embedded rat heart using BAG3 Rabbit pAb (A14826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BAG3 Rabbit pAb (A14826)

Immunohistochemistry analysis of paraffin-embedded human lung using BAG3 Rabbit pAb (A14826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BAG3 Rabbit pAb (A14826)

Immunohistochemistry analysis of paraffin-embedded mouse heart using BAG3 Rabbit pAb (A14826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameBAG3 Rabbit pAb
Catalog No.A14826
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).
SequencePSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP
Gene ID
Swiss Prot
SynonymsBIS; MFM6; BAG-3; CAIR-1; BAG3
Calculated MW62kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA-549, HeLa, HepG2, Mouse brain, Mouse testis
Cellular locationcytoplasm, cytosol, nucleus, Z disc
Customer validation

WB(Rattus norvegicus)

IHC(Rattus norvegicus)

IF(Rattus norvegicus)

Co-IP(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - BAG3 Rabbit pAb (A14826)}

Western blot - BAG3 Rabbit pAb (A14826)

Western blot analysis of various lysates using BAG3 Rabbit pAb (A14826) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BAG3 Rabbit pAb (A14826)}

Immunohistochemistry - BAG3 Rabbit pAb (A14826)

Immunohistochemistry analysis of paraffin-embedded rat heart using BAG3 Rabbit pAb (A14826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BAG3 Rabbit pAb (A14826)}

Immunohistochemistry - BAG3 Rabbit pAb (A14826)

Immunohistochemistry analysis of paraffin-embedded human lung using BAG3 Rabbit pAb (A14826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BAG3 Rabbit pAb (A14826)}

Immunohistochemistry - BAG3 Rabbit pAb (A14826)

Immunohistochemistry analysis of paraffin-embedded mouse heart using BAG3 Rabbit pAb (A14826) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14826? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)