제품 > 항체 > 다중클론항체(pAb)

BAK1 Rabbit pAb (A21283)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Arabidopsis thaliana

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using BAK1 Rabbit pAb (A21283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product name	BAK1 Rabbit pAb
Catalog No.	A21283
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Leu-rich receptor Serine/threonine protein kinase. Component of BR signaling that interacts with BRI1 in vitro and in vivo to form a heterodimer. Brassinolide-dependent association of BRI1 and BAK1 in vivo. Phosphorylation of both BRI1 and BAK1 on Thr residues was BR dependent. Although BAK1 and BRI1 alone localize in the plasma membrane, when BAK1 and BRI1 are coexpressed, the heterodimer BAK1/BRI1 they form is localized in the endosome.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 516-615 of arabidopsis thaliana BAK1 (NP_567920.1).
Sequence	KKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR
Gene ID
Swiss Prot
Synonyms	ATBAK1; ATSERK3; BRI1-associated receptor kinase; ELG; ELONGATED; F17M5.190; F17M5_190; RECEPTOR KINASES LIKE SERK 10; RKS10; SERK3; SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3; BAK1
Calculated MW	68kDa
Observed MW

Applications

Reactivity	Arabidopsis thaliana
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Seedling, Inflorescences, Before inflorescence, Rosette leaf
Cellular location	plasma membrane