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BAP31 Rabbit mAb (A2259)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BAP31 Rabbit mAb (A2259)

Western blot analysis of various lysates using BAP31 Rabbit mAb (A2259) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - BAP31 Rabbit mAb (A2259)

Western blot analysis of various lysates using BAP31 Rabbit mAb (A2259) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - BAP31 Rabbit mAb (A2259)

Immunohistochemistry analysis of paraffin-embedded Human brain using BAP31 Rabbit mAb (A2259) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - BAP31 Rabbit mAb (A2259)

Immunofluorescence analysis of U-2 OS cells using BAP31 Rabbit mAb (A2259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBAP31 Rabbit mAb
Catalog No.A2259
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1894
This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 101-200 of human BAP31 (P51572).
SequenceRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQKLKDELASTKQ
Gene ID
Swiss Prot
SynonymsCDM; DDCH; BAP31; 6C6-AG; DELXQ28; DXS1357E; MICRODELXq28
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, U-251MG, Mouse lung, Mouse liver, Mouse kidney, Rat lung
Cellular locationEndoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Multi-pass membrane protein.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - BAP31 Rabbit mAb (A2259)}

    Western blot - BAP31 Rabbit mAb (A2259)

    Western blot analysis of various lysates using BAP31 Rabbit mAb (A2259) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - BAP31 Rabbit mAb (A2259)}

    Western blot - BAP31 Rabbit mAb (A2259)

    Western blot analysis of various lysates using BAP31 Rabbit mAb (A2259) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - BAP31 Rabbit mAb (A2259)}

    Immunohistochemistry - BAP31 Rabbit mAb (A2259)

    Immunohistochemistry analysis of paraffin-embedded Human brain using BAP31 Rabbit mAb (A2259) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - BAP31 Rabbit mAb (A2259)}

    Immunofluorescence - BAP31 Rabbit mAb (A2259)

    Immunofluorescence analysis of U-2 OS cells using BAP31 Rabbit mAb (A2259) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)