제품 > 항체 > 다중클론항체(pAb)

BC12 Rabbit pAb (A20638)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - BC12 Rabbit pAb (A20638)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using BC12 Rabbit pAb (A20638) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

Overview

Product nameBC12 Rabbit pAb
Catalog No.A20638
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Microtubule-dependent motor protein involved in the control of the oriented deposition of cellulose microfibrils.Involved in wall biogenesis and modification, and contributes to cell-cycle progression and cell division. Acts as a transcriptional activator in gibberellic acid (GA) biosynthesis pathway. Binds specifically to the DNA sequence 5'-ACCAACTTGAA-3' of the ent-kaurene oxidase 2 (CYP701A6 or OsKO2) promoter. May regulate CYP701A6 gene expression and mediates cell elongation by regulating the GA biosynthesis pathway
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 840-970 of Oryza sativa BC12. (Q6YUL8).
SequenceEAKSLLQYIFNVAADARCQVREKEMEIKEMKEQMTELVTILRHSESRRRETEKQLKQREQAAVTATTSPGNGNGSVKHSADDSNTPLSPVAVPAQKQLKYSAGIVNSPSKGVPAFNKQHLKMVPMAQLPVG
Gene ID
Swiss Prot
SynonymsBC2; GDD1; KIF4-like; OsJ_28385; OJ1134_E08.39-1; OJ1134_E08.39-2; OJ1134_E08.39-3; BC12
Calculated MW116kDa
Observed MW116kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesStems
Cellular locationcytoplasm, cytoskeleton, nucleus
    ABclonal:Western blot - BC12 Rabbit pAb (A20638)}

    Western blot - BC12 Rabbit pAb (A20638)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using BC12 Rabbit pAb (A20638) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)