제품 > 항체 > 다중클론항체(pAb)

BCAN Rabbit pAb (A9368)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - BCAN Rabbit pAb (A9368)

Western blot analysis of various lysates using BCAN Rabbit pAb (A9368) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameBCAN Rabbit pAb
Catalog No.A9368
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the lectican family of chondroitin sulfate proteoglycans that is specifically expressed in the central nervous system. This protein is developmentally regulated and may function in the formation of the brain extracellular matrix. This protein is highly expressed in gliomas and may promote the growth and cell motility of brain tumor cells. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 762-911 of human BCAN (NP_068767.3).
SequenceVPLLYENWNPGQPDSYFLSGENCVVMVWHDQGQWSDVPCNYHLSYTCKMGLVSCGPPPELPLAQVFGRPRLRYEVDTVLRYRCREGLAQRNLPLIRCQENGRWEAPQISCVPRRPARALHPEEDPEGRQGRLLGRWKALLIPPSSPMPGP
Gene ID
Swiss Prot
SynonymsBEHAB; CSPG7; BCAN
Calculated MW99kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, K-562, Raji, HeLa, Mouse brain
Cellular locationGPI-anchor, Lipid-anchor, Membrane, Secreted, extracellular matrix, extracellular space
ABclonal:Western blot - BCAN Rabbit pAb (A9368)}

Western blot - BCAN Rabbit pAb (A9368)

Western blot analysis of various lysates using BCAN Rabbit pAb (A9368) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)