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BCAP29 Rabbit pAb (A6335)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BCAP29 Rabbit pAb (A6335)

Western blot analysis of various lysates using BCAP29 Rabbit pAb (A6335) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - BCAP29 Rabbit pAb (A6335)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using BCAP29 Rabbit pAb (A6335) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BCAP29 Rabbit pAb (A6335)

Immunohistochemistry analysis of paraffin-embedded Human stomach using BCAP29 Rabbit pAb (A6335) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BCAP29 Rabbit pAb (A6335)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using BCAP29 Rabbit pAb (A6335) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameBCAP29 Rabbit pAb
Catalog No.A6335
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in osteoblast differentiation. Located in membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 125-241 of human BCAP29 (NP_061332.2).
SequenceTQLAKELSNKGVLKTQAENTNKAAKKFMEENEKLKRILKSHGKDEECVLEAENKKLVEDQEKLKTELRKTSDALSKAQNDVMEMKMQSERLSKEYDQLLKEHSELQDRLERGNKKRL
Gene ID
Swiss Prot
SynonymsB29; BAP29; BCAP29
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSW480, SKOV3, MCF7, THP-1, ES-2, Mouse testis, Mouse heart, Mouse brain, Rat heart
Cellular locationEndoplasmic reticulum membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - BCAP29 Rabbit pAb (A6335)}

    Western blot - BCAP29 Rabbit pAb (A6335)

    Western blot analysis of various lysates using BCAP29 Rabbit pAb (A6335) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - BCAP29 Rabbit pAb (A6335)}

    Immunohistochemistry - BCAP29 Rabbit pAb (A6335)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using BCAP29 Rabbit pAb (A6335) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - BCAP29 Rabbit pAb (A6335)}

    Immunohistochemistry - BCAP29 Rabbit pAb (A6335)

    Immunohistochemistry analysis of paraffin-embedded Human stomach using BCAP29 Rabbit pAb (A6335) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - BCAP29 Rabbit pAb (A6335)}

    Immunohistochemistry - BCAP29 Rabbit pAb (A6335)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen using BCAP29 Rabbit pAb (A6335) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)