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BCL3 Rabbit pAb (A11582)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BCL3 Rabbit pAb (A11582)

Western blot analysis of various lysates using BCL3 Rabbit pAb (A11582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - BCL3 Rabbit pAb (A11582)

Immunofluorescence analysis of NIH/3T3 cells using BCL3 Rabbit pAb (A11582) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - BCL3 Rabbit pAb (A11582)

Immunofluorescence analysis of HeLa cells using BCL3 Rabbit pAb (A11582) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - BCL3 Rabbit pAb (A11582)

Immunofluorescence analysis of C6 cells using BCL3 Rabbit pAb (A11582) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBCL3 Rabbit pAb
Catalog No.A11582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human BCL3 (NP_005169.2).
SequenceANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPS
Gene ID
Swiss Prot
SynonymsBCL4; D19S37; BCL3
Calculated MW48kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRaji, 293T
Cellular locationCytoplasm, Nucleus, perinuclear region
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - BCL3 Rabbit pAb (A11582)}

Western blot - BCL3 Rabbit pAb (A11582)

Western blot analysis of various lysates using BCL3 Rabbit pAb (A11582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - BCL3 Rabbit pAb (A11582)}

Immunofluorescence - BCL3 Rabbit pAb (A11582)

Immunofluorescence analysis of NIH/3T3 cells using BCL3 Rabbit pAb (A11582) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BCL3 Rabbit pAb (A11582)}

Immunofluorescence - BCL3 Rabbit pAb (A11582)

Immunofluorescence analysis of HeLa cells using BCL3 Rabbit pAb (A11582) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BCL3 Rabbit pAb (A11582)}

Immunofluorescence - BCL3 Rabbit pAb (A11582)

Immunofluorescence analysis of C6 cells using BCL3 Rabbit pAb (A11582) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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ELISA 키트 (1)

Secondary Antibodies (26)