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BHMT Rabbit mAb (A23448)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BHMT Rabbit mAb (A23448)

Western blot analysis of various lysates, using BHMT Rabbit mAb (A23448) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - BHMT Rabbit mAb (A23448)

Western blot analysis of lysates from HepG2 cells, using BHMT Rabbit mAb (A23448) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - BHMT Rabbit mAb (A23448)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using BHMT Rabbit mAb (A23448) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BHMT Rabbit mAb (A23448)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using BHMT Rabbit mAb (A23448) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameBHMT Rabbit mAb
Catalog No.A23448
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58111
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 306-406 of human BHMT. (NP_001704.2).
SequenceHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ
Gene ID
Swiss Prot
SynonymsBHMT1; HEL-S-61p; BHMT
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHep G2, Rat liver
Cellular locationCytoplasm

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ABclonal:Western blot - BHMT Rabbit mAb (A23448)}

Western blot - BHMT Rabbit mAb (A23448)

Western blot analysis of various lysates, using BHMT Rabbit mAb (A23448) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - BHMT Rabbit mAb (A23448)}

Western blot - BHMT Rabbit mAb (A23448)

Western blot analysis of lysates from HepG2 cells, using BHMT Rabbit mAb (A23448) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - BHMT Rabbit mAb (A23448)}

Immunohistochemistry - BHMT Rabbit mAb (A23448)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using BHMT Rabbit mAb (A23448) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - BHMT Rabbit mAb (A23448)}

Immunohistochemistry - BHMT Rabbit mAb (A23448)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using BHMT Rabbit mAb (A23448) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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