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BIN1 Rabbit mAb (A21108)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BIN1 Rabbit mAb (A21108)

Western blot analysis of various lysates, using BIN1 Rabbit mAb (A21108) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.

ABclonal:Immunohistochemistry - BIN1 Rabbit mAb (A21108)

Immunohistochemistry analysis of paraffin-embedded mouse brain using BIN1 Rabbit mAb (A21108) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BIN1 Rabbit mAb (A21108)

Immunohistochemistry analysis of paraffin-embedded rat brain using BIN1 Rabbit mAb (A21108) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameBIN1 Rabbit mAb
Catalog No.A21108
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3066
This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 400-593 of human BIN1 (NP_647601.1).
SequencePVTSPVKAPTPSGQSIPWDLWEPTESPAGSLPSGEPSAAEGTFAVSWPSQTAEPGPAQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP
Gene ID
Swiss Prot
SynonymsCNM2; AMPH2; AMPHL; SH3P9; BIN1
Calculated MW65kDa
Observed MW45-80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, U-87MG, Mouse skeletal muscle
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - BIN1 Rabbit mAb (A21108)}

Western blot - BIN1 Rabbit mAb (A21108)

Western blot analysis of various lysates, using BIN1 Rabbit mAb (A21108) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.
ABclonal:Immunohistochemistry - BIN1 Rabbit mAb (A21108)}

Immunohistochemistry - BIN1 Rabbit mAb (A21108)

Immunohistochemistry analysis of paraffin-embedded mouse brain using BIN1 Rabbit mAb (A21108) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BIN1 Rabbit mAb (A21108)}

Immunohistochemistry - BIN1 Rabbit mAb (A21108)

Immunohistochemistry analysis of paraffin-embedded rat brain using BIN1 Rabbit mAb (A21108) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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