제품 > 항체 > 다중클론항체(pAb)

BIN2 Rabbit pAb (A21175)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Arabidopsis thaliana

Western blot analysis of lysates from Rosette leaves, using BIN2 Rabbit pAb (A21175) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	BIN2 Rabbit pAb
Catalog No.	A21175
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Encodes BIN2, a member of the ATSK (shaggy-like kinase) family. BIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. BIN2 regulates root epidermal cell fate specification by phosphorylating EGL3 and TTG1. BIN2-mediated phosphorylation appears to promote BZR1 export from the nucleus.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 281-380 of arabidopsis thaliana BIN2 (NP_193606.1).
Sequence	KAHPWHKIFHKRMPPEAIDFASRLLQYSPSLRCTALEACAHPFFDELREPNARLPNGRPFPPLFNFKQEVAGSSPELVNKLIPDHIKRQLGLSFLNQSGT
Gene ID
Swiss Prot
Synonyms	ATSK21; BRASSINOSTEROID-INSENSITIVE 2; DWARF 12; DWF12; F28A21.120; F28A21_120; SHAGGY-LIKE KINASE 21; SK21; UCU1; ULTRACURVATA 1; BIN2
Calculated MW	43kDa
Observed MW	43kDa

Applications

Reactivity	Arabidopsis thaliana
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Rosette leaves
Cellular location	cell cortex, cytoplasm, nucleus, plasma membrane