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BLVRB Rabbit pAb (A7260)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BLVRB Rabbit pAb (A7260)

Western blot analysis of various lysates using BLVRB Rabbit pAb (A7260) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - BLVRB Rabbit pAb (A7260)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using BLVRB Rabbit pAb (A7260) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BLVRB Rabbit pAb (A7260)

Immunohistochemistry analysis of paraffin-embedded Human stomach using BLVRB Rabbit pAb (A7260) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameBLVRB Rabbit pAb
Catalog No.A7260
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables biliverdin reductase (NAD(P)+) activity and riboflavin reductase (NADPH) activity. Involved in heme catabolic process. Located in cytosol; nucleoplasm; and plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human BLVRB (NP_000704.1).
SequenceMAVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVVVGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ
Gene ID
Swiss Prot
SynonymsFLR; BVRB; SDR43U1; HEL-S-10; BLVRB
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, U-251MG
Cellular locationCytoplasm
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - BLVRB Rabbit pAb (A7260)}

    Western blot - BLVRB Rabbit pAb (A7260)

    Western blot analysis of various lysates using BLVRB Rabbit pAb (A7260) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - BLVRB Rabbit pAb (A7260)}

    Immunohistochemistry - BLVRB Rabbit pAb (A7260)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using BLVRB Rabbit pAb (A7260) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - BLVRB Rabbit pAb (A7260)}

    Immunohistochemistry - BLVRB Rabbit pAb (A7260)

    Immunohistochemistry analysis of paraffin-embedded Human stomach using BLVRB Rabbit pAb (A7260) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A7260? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)