제품 > 항체 > 다중클론항체(pAb)

BLZF1 Rabbit pAb (A6430)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - BLZF1 Rabbit pAb (A6430)

Western blot analysis of extracts of various cell lines, using BLZF1 antibody (A6430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - BLZF1 Rabbit pAb (A6430)

Immunofluorescence analysis of U2OS cells using BLZF1 antibody (A6430). Blue: DAPI for nuclear staining.

Overview

Product nameBLZF1 Rabbit pAb
Catalog No.A6430
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ubiquitin protein ligase binding activity. Acts upstream of or within Golgi organization and Golgi to plasma membrane protein transport. Located in Golgi apparatus and nucleoplasm. Biomarker of hepatocellular carcinoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1).
SequenceMTTKNLETKVTVTSSPIRGAGDGMETEEPPKSVEVTSGVQSRKHHSLQSPWKKAVPSESPGVLQLGKMLTEKAMEVKAVRILVPKAAITHDIPNKNTKVKSLGHHKGEFLGQSEGVIEPNKELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNQLILENEALGRNTAQLSEQLERMSIQCDVWRSKFLASRVMADELTNSRAALQRQNRDAHGAIQDLLSEREQFRQEMIATQKLLEELLVSLQWGREQTYSPSVQPHSTAELALTNHKLAKAVNSHLLGNVGINNQKKIPSTVEFCSTPAEKMAETVLRILDPVTCKESSPDNPFFESSPTTLLATKKNIGRFHPYTRYENITFNCCNHCRGELIAL
Gene ID
Swiss Prot
SynonymsJEM1; JEM-1; JEM-1s; GOLGIN-45
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, NIH/3T3, U-251MG, Jurkat, Mouse skeletal muscle
Cellular locationCytoplasm, Golgi apparatus lumen, Nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - BLZF1 Rabbit pAb (A6430)}

    Western blot - BLZF1 Rabbit pAb (A6430)

    Western blot analysis of extracts of various cell lines, using BLZF1 antibody (A6430) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - BLZF1 Rabbit pAb (A6430)}

    Immunofluorescence - BLZF1 Rabbit pAb (A6430)

    Immunofluorescence analysis of U2OS cells using BLZF1 antibody (A6430). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)