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BNIP1 Rabbit mAb (A4405)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BNIP1 Rabbit mAb (A4405)

Western blot analysis of various lysates using BNIP1 Rabbit mAb (A4405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - BNIP1 Rabbit mAb (A4405)

Immunohistochemistry analysis of paraffin-embedded rat brain using BNIP1 Rabbit mAb (A4405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BNIP1 Rabbit mAb (A4405)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using BNIP1 Rabbit mAb (A4405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameBNIP1 Rabbit mAb
Catalog No.A4405
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2137
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human BNIP1 (Q12981).
SequenceKFQQLRHRIQDLEQLAKEQDKESEKQLLLQEVENHKKQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTSSTITESLMGISRMMA
Gene ID
Swiss Prot
SynonymsNIP1; SEC20; TRG-8; BNIP1
Calculated MW26kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse heart, Rat heart
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrial membrane, Nuclear envelope, SNARE complex

Documents

Certificate of Compliance

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    ABclonal:Western blot - BNIP1 Rabbit mAb (A4405)}

    Western blot - BNIP1 Rabbit mAb (A4405)

    Western blot analysis of various lysates using BNIP1 Rabbit mAb (A4405) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - BNIP1 Rabbit mAb (A4405)}

    Immunohistochemistry - BNIP1 Rabbit mAb (A4405)

    Immunohistochemistry analysis of paraffin-embedded rat brain using BNIP1 Rabbit mAb (A4405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - BNIP1 Rabbit mAb (A4405)}

    Immunohistochemistry - BNIP1 Rabbit mAb (A4405)

    Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using BNIP1 Rabbit mAb (A4405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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