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BRCC2/BLID Rabbit pAb (A7275)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BRCC2/BLID Rabbit pAb (A7275)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BRCC2/BLID, using BRCC2/BLID Rabbit pAb (A7275) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - BRCC2/BLID Rabbit pAb (A7275)

Immunofluorescence analysis of MCF-7 cells using BRCC2/BLID Rabbit pAb (A7275). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBRCC2/BLID Rabbit pAb
Catalog No.A7275
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a BH3-like motif containing protein involved in cell death. The encoded protein may induce apoptosis in a caspase-dependent manner. The protein is localized in both the cytoplasm and the mitochondrion.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human BRCC2/BLID (NP_001001786.2).
SequenceMVTLLPIEGQEIHFFEILESECVLYTGWIERASGSSIYPEAKARLPLEALLGSNKEPMLPKETVLSLKRYNLGSSAMKRNVPGHVLQRPSYLTRIQVTLLCNSSAEAL
Gene ID
Swiss Prot
SynonymsBRCC2; BRCC2/BLID
Calculated MW12kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T transfected with BRCC2/BLID
Cellular locationCytoplasm, Mitochondrion.

Documents

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ABclonal:Western blot - BRCC2/BLID Rabbit pAb (A7275)}

Western blot - BRCC2/BLID Rabbit pAb (A7275)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BRCC2/BLID, using BRCC2/BLID Rabbit pAb (A7275) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - BRCC2/BLID Rabbit pAb (A7275)}

Immunofluorescence - BRCC2/BLID Rabbit pAb (A7275)

Immunofluorescence analysis of MCF-7 cells using BRCC2/BLID Rabbit pAb (A7275). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)