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BRD3 Rabbit pAb (A2277)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - BRD3 Rabbit pAb (A2277)

Western blot analysis of various lysates using BRD3 Rabbit pAb (A2277) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - BRD3 Rabbit pAb (A2277)

Immunohistochemistry analysis of paraffin-embedded Rat testis using BRD3 Rabbit pAb (A2277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameBRD3 Rabbit pAb
Catalog No.A2277
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 150-305 of human BRD3 (NP_031397.1).
SequencePAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKK
Gene ID
Swiss Prot
SynonymsORFX; FSHRG2; RING3L; BRD3
Calculated MW80kDa
Observed MW115kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, HeLa, HepG2, DU145, BT-474
Cellular locationNucleus.
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - BRD3 Rabbit pAb (A2277)}

    Western blot - BRD3 Rabbit pAb (A2277)

    Western blot analysis of various lysates using BRD3 Rabbit pAb (A2277) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - BRD3 Rabbit pAb (A2277)}

    Immunohistochemistry - BRD3 Rabbit pAb (A2277)

    Immunohistochemistry analysis of paraffin-embedded Rat testis using BRD3 Rabbit pAb (A2277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A2277? Please let us know so that we can cite the reference in this datasheet.