제품 > 항체 > 다중클론항체(pAb)

BSU1 Rabbit pAb (A21273)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - BSU1 Rabbit pAb (A21273)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using BSU1 Rabbit pAb (A21273) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameBSU1 Rabbit pAb
Catalog No.A21273
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
encodes a serine#Aeithreonine protein phosphatase with an N-terminal Kelch-repeat domain, which is nuclear localized and expressed preferentially in elongating cells. Genetic evidence suggest that this gene plays a redundant role (along with other members of the same gene family) in modulating growth in response to brassinosteroid.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 694-793 of arabidopsis thaliana BSU1 (NP_171844.6).
SequenceFLERNGLEMILRAHECVIDGFERFADGRLITVFSATNYCGTAQNAGAILVIGRDMVIYPKLIHPHPPPISSSEEDYTDKAWMQELNIEMPPTPARGESSE
Gene ID
Swiss Prot
SynonymsBRI1 SUPPRESSOR 1; F21B7.7; BSU1
Calculated MW88kDa
Observed MW88kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSeedling, Inflorescences, Before inflorescence
Cellular locationcytoplasm, nucleus, plasma membrane

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    ABclonal:Western blot - BSU1 Rabbit pAb (A21273)}

    Western blot - BSU1 Rabbit pAb (A21273)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using BSU1 Rabbit pAb (A21273) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.