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BTD Rabbit pAb (A6284)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BTD Rabbit pAb (A6284)

Western blot analysis of various lysates using BTD Rabbit pAb (A6284) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BTD Rabbit pAb (A6284)

Immunohistochemistry analysis of paraffin-embedded Rat brain using BTD Rabbit pAb (A6284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BTD Rabbit pAb (A6284)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using BTD Rabbit pAb (A6284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BTD Rabbit pAb (A6284)

Immunohistochemistry analysis of paraffin-embedded Human prostate using BTD Rabbit pAb (A6284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameBTD Rabbit pAb
Catalog No.A6284
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 284-543 of human BTD (NP_000051.1).
SequenceIQKAFAVAFGINVLAANVHHPVLGMTGSGIHTPLESFWYHDMENPKSHLIIAQVAKNPVGLIGAENATGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNAPPTFHSEMMYDNFTLVPVWGKEGYLHVCSNGLCCYLLYERPTLSKELYALGVFDGLHTVHGTYYIQVCALVRCGGLGFDTCGQEITEATGIFEFHLWGNFSTSYIFPLFLTSGMTLEVPDQLGWENDHYFLRKSRLSSGLVTAALYGRLYERD
Gene ID
Swiss Prot
SynonymsBTD
Calculated MW61kDa
Observed MW61kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse liver, Mouse kidney
Cellular locationSecreted, extracellular space

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - BTD Rabbit pAb (A6284)}

    Western blot - BTD Rabbit pAb (A6284)

    Western blot analysis of various lysates using BTD Rabbit pAb (A6284) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - BTD Rabbit pAb (A6284)}

    Immunohistochemistry - BTD Rabbit pAb (A6284)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using BTD Rabbit pAb (A6284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - BTD Rabbit pAb (A6284)}

    Immunohistochemistry - BTD Rabbit pAb (A6284)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using BTD Rabbit pAb (A6284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - BTD Rabbit pAb (A6284)}

    Immunohistochemistry - BTD Rabbit pAb (A6284)

    Immunohistochemistry analysis of paraffin-embedded Human prostate using BTD Rabbit pAb (A6284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)