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BTNL2 Rabbit pAb (A7120)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BTNL2 Rabbit pAb (A7120)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BTNL2 using BTNL2 Rabbit pAb (A7120) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - BTNL2 Rabbit pAb (A7120)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using BTNL2 Rabbit pAb (A7120) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameBTNL2 Rabbit pAb
Catalog No.A7120
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a major histocompatibility complex, class II associated, type I transmembrane protein which belongs to the butyrophilin-like B7 family of immunoregulators. It is thought to be involved in immune surveillance, serving as a negative T-cell regulator by decreasing T-cell proliferation and cytokine release. The encoded protein contains an N-terminal signal peptide, two pairs of immunoglobulin-like domains, separated by a heptad peptide sequence, and a C-terminal transmembrane domain. Naturally occurring mutations in this gene are associated with sarcoidosis, rheumatoid arthritis, ulcerative colitis, inflammatory bowel disease, myositis, type 1 diabetes, systemic lupus erythematosus, acute coronary syndrome, and prostate cancer.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 246-455 of human BTNL2 (NP_062548.1).
SequenceLKVNGPSQPILVRVGEDIQLTCYLSPKANAQSMEVRWDRSHRYPAVHVYMDGDHVAGEQMAEYRGRTVLVSDAIDEGRLTLQILSARPSDDGQYRCLFEKDDVYQEASLDLKVVSLGSSPLITVEGQEDGEMQPMCSSDGWFPQPHVPWRDMEGKTIPSSSQALTQGSHGLFHVQTLLRVTNISAVDVTCSISIPFLGEEKIATFSLSGW
Gene ID
Swiss Prot
SynonymsSS2; BTN7; BTL-II; HSBLMHC1; BTNL2
Calculated MW50kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T transfected with BTNL2
Cellular locationMembrane, Single-pass type II membrane protein.

Documents

Certificate of Compliance

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    ABclonal:Western blot - BTNL2 Rabbit pAb (A7120)}

    Western blot - BTNL2 Rabbit pAb (A7120)

    Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BTNL2 using BTNL2 Rabbit pAb (A7120) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - BTNL2 Rabbit pAb (A7120)}

    Immunohistochemistry - BTNL2 Rabbit pAb (A7120)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using BTNL2 Rabbit pAb (A7120) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)