Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using BUB3 Rabbit mAb (A8831) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of BUB3 in paraffin-embedded rat spleen tissue using BUB3 Rabbit mAb (A8831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of BUB3 in paraffin-embedded human colon carcinoma tissue using BUB3 Rabbit mAb (A8831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of BUB3 in paraffin-embedded mouse brain tissue using BUB3 Rabbit mAb (A8831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of BUB3 in paraffin-embedded rat brain tissue using BUB3 Rabbit mAb (A8831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of BUB3 in paraffin-embedded rat colon tissue using BUB3 Rabbit mAb (A8831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of BUB3 in paraffin-embedded human colon tissue using BUB3 Rabbit mAb (A8831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of BUB3 in paraffin-embedded mouse lung tissue using BUB3 Rabbit mAb (A8831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using BUB3 Rabbit mAb (A8831) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | BUB3 Rabbit mAb |
|---|---|
| Catalog No. | A8831 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1318 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BUB3 (O43684). |
|---|---|
| Sequence | MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIR |
| Gene ID | |
| Swiss Prot | |
| Synonyms | BUB3L; hBUB3; BUB3 |
| Calculated MW | 37kDa |
| Observed MW |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HeLa, 293T, MCF7, C2C12, Mouse liver, Rat lung |
| Cellular location | Chromosome, Nucleus, centromere, kinetochore |
| Customer validation | WB(Mus musculus, Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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