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Beta-galactosidase （GLB1）Rabbit mAb (A23753)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates, using Beta-galactosidase （GLB1）Rabbit mAb (A23753) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Beta-galactosidase （GLB1）Rabbit mAb (A23753)

Immunohistochemistry analysis of paraffin-embedded human liver using Beta-galactosidase （GLB1）Rabbit mAb (A23753) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Beta-galactosidase （GLB1）Rabbit mAb (A23753) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	Beta-galactosidase （GLB1）Rabbit mAb
Catalog No.	A23753
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61596

Background

This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.

Immunogen information

Immunogen	Recombinant protein of human Beta-galactosidase （GLB1）.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	GLB1; EBP; ELNR1; MPS4B; beta-galactosidase
Calculated MW	60kDa/72kDa/76kDa
Observed MW	76kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HCT116, 293F
Cellular location	Cytoplasm, Lysosome, perinuclear region