제품 > 항체 > 다중클론항체(pAb)

C10orf32 Rabbit pAb (A7276)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - C10orf32 Rabbit pAb (A7276)

Western blot analysis of Rat brain, using C10orf32 Rabbit pAb (A7276) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - C10orf32 Rabbit pAb (A7276)

Immunofluorescence analysis of MCF-7 cells using C10orf32 antibody (A7276). Blue: DAPI for nuclear staining.

Overview

Product nameC10orf32 Rabbit pAb
Catalog No.A7276
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Part of BORC complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human C10orf32 (NP_653192.2).
SequenceMMATGTPESQARFGQSVKGLLTEKVTTCGTDVIALTKQVLKGSRSSELLGQAARNMVLQEDAILHSEDSLRKMAIITTHLQYQQEAIQKNVEQSSDLQDQLNHLL
Gene ID
Swiss Prot
SynonymsC10orf32
Calculated MW12kDa
Observed MW18kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationBORC complex

Documents

Certificate of Compliance

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    ABclonal:Western blot - C10orf32 Rabbit pAb (A7276)}

    Western blot - C10orf32 Rabbit pAb (A7276)

    Western blot analysis of Rat brain, using C10orf32 Rabbit pAb (A7276) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - C10orf32 Rabbit pAb (A7276)}

    Immunofluorescence - C10orf32 Rabbit pAb (A7276)

    Immunofluorescence analysis of MCF-7 cells using C10orf32 antibody (A7276). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)