제품 > 항체 > 다중클론항체(pAb)

C1QL1 Rabbit pAb (A18419)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using C1QL1 Rabbit pAb (A18419) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	C1QL1 Rabbit pAb
Catalog No.	A18419
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable signaling receptor binding activity. Predicted to act upstream of or within maintenance of synapse structure; motor learning; and neuron remodeling. Predicted to be located in several cellular components, including climbing fiber; presynapse; and synaptic cleft. Predicted to be part of collagen trimer.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 38-88 of human C1QL1 (NP_006679.1).
Sequence	RGPGAGARTDGGDALSEQSGAPPPSTLVQGPQGKPGRTGKPGPPGPPGDPG
Gene ID
Swiss Prot
Synonyms	CRF; C1QRF; CTRP14; C1QTNF14; C1QL1
Calculated MW	26kDa
Observed MW	26kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	SH-SY5Y, Mouse brain, Rat brain
Cellular location	cerebellar climbing fiber to Purkinje cell synapse, climbing fiber, cytoplasm, extracellular space, presynapse, synaptic cleft