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C3 Rabbit pAb (A16781)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - C3 Rabbit pAb (A16781)

Western blot analysis of lysates from Rat lung, using C3 Rabbit pAb (A16781) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameC3 Rabbit pAb
Catalog No.A16781
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1159-1286 of mouse C3 (NP_033908.2).
SequenceEGQVNSLPGSINKAGEYIEASYMNLQRPYTVAIAGYALALMNKLEEPYLGKFLNTAKDRNRWEEPDQQLYNVEATSYALLALLLLKDFDSVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQTDV
Gene ID
Swiss Prot
SynonymsASP; Plp; HSE-MSF; C3
Calculated MW186kDa
Observed MW115kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat lung
Cellular locationcell surface, extracellular region, extracellular space, plasma membrane.
Customer validation

IHC(Mus musculus)

IF(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - C3 Rabbit pAb (A16781)}

    Western blot - C3 Rabbit pAb (A16781)

    Western blot analysis of lysates from Rat lung, using C3 Rabbit pAb (A16781) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16781? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

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    Secondary Antibodies (26)