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Complement C3 Rabbit mAb (A22265)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Complement C3 Rabbit mAb (A22265)

Western blot analysis of lysates from Mouse liver, using Complement C3 Rabbit mAb (A22265) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Complement C3 Rabbit mAb (A22265)

Western blot analysis of various lysates, using Complement C3 Rabbit mAb (A22265) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameComplement C3 Rabbit mAb
Catalog No.A22265
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56851
This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of mouse Complement C3 (NP_033908.2).
SequenceRVSIRPAPETAKKPEEAKNTMFLEICTKYLGDVDATMSILDISMMTGFAPDTKDLELLASGVDRYISKYEMNKAFSNKNTLIIYLEKISHTEEDCLTFKVHQYFNVGLIQPGSVKVYSYYNLEESCTRFYHPEKDDGMLSKLCHSEMCRCAEENCFMQQSQEKINLNVRLDKACEPGVDYVYKTELTNIELLDDFDEYTMTIQQVIKSGSDEVQAGQQRKFISHIKCRNALKLQKGKKYLMWGLSSDLWGEKPNTSYIIGKDTWVEHWPEAEECQDQKYQKQCEELGAFTESMVVYGCPN
Gene ID
Swiss Prot
SynonymsASP; Plp; HSE-MSF; Complement C3
Calculated MW186kDa
Observed MW115kDa/187kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver, Rat liver, Rat lung
Cellular locationCell surface, Extracellular region, Extracellular space, Plasma membrane.
Customer validation

WB(Other, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Complement C3 Rabbit mAb (A22265)}

    Western blot - Complement C3 Rabbit mAb (A22265)

    Western blot analysis of lysates from Mouse liver, using Complement C3 Rabbit mAb (A22265) at1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Complement C3 Rabbit mAb (A22265)}

    Western blot - Complement C3 Rabbit mAb (A22265)

    Western blot analysis of various lysates, using Complement C3 Rabbit mAb (A22265) at1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A22265? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    재조합 단백질 (1)

    Secondary Antibodies (26)