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CACNG1 Rabbit pAb (A10014)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunofluorescence - CACNG1 Rabbit pAb (A10014)

Immunofluorescence analysis of C6 cells using CACNG1 Rabbit pAb (A10014) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CACNG1 Rabbit pAb (A10014)

Immunofluorescence analysis of L929 cells using CACNG1 Rabbit pAb (A10014) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CACNG1 Rabbit pAb (A10014)

Immunofluorescence analysis of SH-SY5Y cells using CACNG1 Rabbit pAb (A10014) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCACNG1 Rabbit pAb
Catalog No.A10014
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Voltage-dependent calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of two known gamma subunit proteins. This particular gamma subunit is part of skeletal muscle 1, 4-dihydropyridine-sensitive calcium channels and is an integral membrane protein that plays a role in excitation-contraction coupling. This gene is part of a functionally diverse eight-member protein subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two family members that function as transmembrane AMPA receptor regulatory proteins (TARPs).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-110 of human CACNG1 (NP_000718.1).
SequenceDHWAVLSPHMEHHNTTCEAAHFGLWRICTKRIPMDDSKTCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAI
Gene ID
Swiss Prot
SynonymsCACNLG
Calculated MW25kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse skeletal muscle, Mouse liver, Mouse kidney, Rat skeletal muscle, Rat liver, Rat kidney
Cellular locationMembrane, Multi-pass membrane protein
Customer validation

WB(Rattus norvegicus)

Documents

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ABclonal:Immunofluorescence - CACNG1 Rabbit pAb (A10014)}

Immunofluorescence - CACNG1 Rabbit pAb (A10014)

Immunofluorescence analysis of C6 cells using CACNG1 Rabbit pAb (A10014) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CACNG1 Rabbit pAb (A10014)}

Immunofluorescence - CACNG1 Rabbit pAb (A10014)

Immunofluorescence analysis of L929 cells using CACNG1 Rabbit pAb (A10014) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CACNG1 Rabbit pAb (A10014)}

Immunofluorescence - CACNG1 Rabbit pAb (A10014)

Immunofluorescence analysis of SH-SY5Y cells using CACNG1 Rabbit pAb (A10014) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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