제품 > 항체 > 다중클론항체(pAb)

CAL Rabbit pAb (A20639)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using CAL Rabbit pAb (A20639) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name	CAL Rabbit pAb
Catalog No.	A20639
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Plant defensin-like protein involved in accumulation of cadmium (Cd) in rice leaves. Mediates Cd efflux from cytosol into extracellular spaces via chelation. This drives Cd secretion from xylem parenchyma cells into the xylem vessels, hence lowering Cd levels in cytosol meanwhile promoting Cd translocation from roots to shoots.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of Oryza sativa CAL. (Q6K209).
Sequence	MAPSRRMVASAFLLLAILVATEMGTTKVAEARHCLSQSHRFKGMCVSSNNCANVCRTESFPDGECKSHGLERKCFCKKVC
Gene ID
Swiss Prot
Synonyms	BC12; CAL
Calculated MW	9kDa
Observed MW	9kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Leaves, Stems
Cellular location	extracellular space