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CAMK1D Rabbit pAb (A7512)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CAMK1D Rabbit pAb (A7512)

Western blot analysis of extracts of various cell lines, using CAMK1D antibody (A7512) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

ABclonal:Immunofluorescence - CAMK1D Rabbit pAb (A7512)

Immunofluorescence analysis of A549 cells using CAMK1D antibody (A7512).

Overview

Product nameCAMK1D Rabbit pAb
Catalog No.A7512
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of the serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It has been associated with multiple processes including regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophil cells, and apoptosis of erythroleukemia cells. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human CAMK1D (NP_705718.1).
SequenceMARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMST
Gene ID
Swiss Prot
SynonymsCKLiK; CaM-K1; CaMKID
Calculated MW43kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-549, 293T, Jurkat, Mouse thymus, Mouse spleen, Rat brain
Cellular locationCytoplasm, Nucleus

Documents

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ABclonal:Western blot - CAMK1D Rabbit pAb (A7512)}

Western blot - CAMK1D Rabbit pAb (A7512)

Western blot analysis of extracts of various cell lines, using CAMK1D antibody (A7512) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
ABclonal:Immunofluorescence - CAMK1D Rabbit pAb (A7512)}

Immunofluorescence - CAMK1D Rabbit pAb (A7512)

Immunofluorescence analysis of A549 cells using CAMK1D antibody (A7512).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (25)