제품 > 항체 > 다중클론항체(pAb)

CAMK1G Rabbit pAb (A7379)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CAMK1G Rabbit pAb (A7379)

Western blot analysis of extracts of various cell lines, using CAMK1G antibody (A7379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - CAMK1G Rabbit pAb (A7379)

Western blot analysis of extracts of various cell lines, using CAMK1G antibody (A7379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameCAMK1G Rabbit pAb
Catalog No.A7379
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable calmodulin binding activity and calmodulin-dependent protein kinase activity. Predicted to be involved in peptidyl-serine phosphorylation. Predicted to be located in endomembrane system. Predicted to be part of calcium- and calmodulin-dependent protein kinase complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 217-476 of human CAMK1G (NP_065172.1).
SequencePPFYEETESKLFEKIKEGYYEFESPFWDDISESAKDFICHLLEKDPNERYTCEKALSHPWIDGNTALHRDIYPSVSLQIQKNFAKSKWRQAFNAAAVVHHMRKLHMNLHSPGVRPEVENRPPETQASETSRPSSPEITITEAPVLDHSVALPALTQLPCQHGRRPTAPGGRSLNCLVNGSLHISSSLVPMHQGSLAAGPCGCCSSCLNIGSKGKSSYCSEPTLLKKANKKQNFKSEVMVPVKASGSSHCRAGQTGVCLIM
Gene ID
Swiss Prot
SynonymsVWS1; CLICK3; CLICKIII; dJ272L16.1
Calculated MW53kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-12, H460, A-673, Mouse brain, Mouse testis, Mouse liver, Rat brain, Rat kidney
Cellular locationCell membrane, Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein

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ABclonal:Western blot - CAMK1G Rabbit pAb (A7379)}

Western blot - CAMK1G Rabbit pAb (A7379)

Western blot analysis of extracts of various cell lines, using CAMK1G antibody (A7379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - CAMK1G Rabbit pAb (A7379)}

Western blot - CAMK1G Rabbit pAb (A7379)

Western blot analysis of extracts of various cell lines, using CAMK1G antibody (A7379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.