제품 > 항체 > 다중클론항체(pAb)

CAPN7 Rabbit pAb (A17666)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CAPN7 Rabbit pAb (A17666)

Western blot analysis of lysates from Jurkat cells, using CAPN7 Rabbit pAb (A17666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - CAPN7 Rabbit pAb (A17666)

Immunofluorescence analysis of A431 cells using CAPN7 Rabbit pAb (A17666) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CAPN7 Rabbit pAb (A17666)

Immunofluorescence analysis of L929 cells using CAPN7 Rabbit pAb (A17666) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCAPN7 Rabbit pAb
Catalog No.A17666
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The function of the protein encoded by this gene is not known. An orthologue has been found in mouse but it seems to diverge from other family members. The mouse orthologue is thought to be calcium independent with protease activity.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human CAPN7 (NP_055111.1).
SequenceRDHEGRYSEAVFYYKEAAQALIYAEMAGSSLENIQEKITEYLERVQALHSAVQSKSADPLKSKHQLDLERAHFLVTQAFDEDEKENVEDAIELYTEAVDLCLKTSYETADKVLQNKLKQLARQALDRAEALSEPLTKPVGKISSTSVKPKPPPVRAHFPLGANPFLERPQSFISPQSCDAQGQRYTAEEIEVLRTTSKING
Gene ID
Swiss Prot
SynonymsPALBH; CALPAIN7; CAPN7
Calculated MW93kDa
Observed MW93kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat
Cellular locationcentrosome, cytosol, extracellular exosome, nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - CAPN7 Rabbit pAb (A17666)}

Western blot - CAPN7 Rabbit pAb (A17666)

Western blot analysis of lysates from Jurkat cells, using CAPN7 Rabbit pAb (A17666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - CAPN7 Rabbit pAb (A17666)}

Immunofluorescence - CAPN7 Rabbit pAb (A17666)

Immunofluorescence analysis of A431 cells using CAPN7 Rabbit pAb (A17666) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CAPN7 Rabbit pAb (A17666)}

Immunofluorescence - CAPN7 Rabbit pAb (A17666)

Immunofluorescence analysis of L929 cells using CAPN7 Rabbit pAb (A17666) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.