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CAVIN2 Rabbit pAb (A23889)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CAVIN2 Rabbit pAb (A23889)

Western blot analysis of various lysates, using CAVIN2 Rabbit pAb (A23889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CAVIN2 Rabbit pAb (A23889)

Immunohistochemistry analysis of paraffin-embedded human spleen using CAVIN2 Rabbit pAb (A23889) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameCAVIN2 Rabbit pAb
Catalog No.A23889
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein is a substrate for protein kinase C (PKC) phosphorylation and recruits polymerase I and transcript release factor (PTRF) to caveolae. Removal of this protein causes caveolae loss and its over-expression results in caveolae deformation and membrane tubulation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 325-425 of human CAVIN2(NP_004648.1).
SequencePNDQEEESFAEGHSEASLASALVEGEIAEEAAEKATSRGSNSGMDSNIDLTIVEDEEEESVALEQAQKVRYEGSYALTSEEAERSDGDPVQPAVLQVHQTS
Gene ID
Swiss Prot
SynonymsSDR; SDPR; PS-p68; cavin-2
Calculated MW47KDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA-549, Mouse heart, Rat lung
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CAVIN2 Rabbit pAb (A23889)}

    Western blot - CAVIN2 Rabbit pAb (A23889)

    Western blot analysis of various lysates, using CAVIN2 Rabbit pAb (A23889) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - CAVIN2 Rabbit pAb (A23889)}

    Immunohistochemistry - CAVIN2 Rabbit pAb (A23889)

    Immunohistochemistry analysis of paraffin-embedded human spleen using CAVIN2 Rabbit pAb (A23889) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (25)