제품 > 항체 > 다중클론항체(pAb)

CCR7 Rabbit pAb (A25849)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - CCR7 Rabbit pAb (A25849)

Western blot analysis of lysates from Rat spleen using CCR7 Rabbit pAb (A25849) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameCCR7 Rabbit pAb
Catalog No.A25849
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables C-C chemokine receptor activity. Involved in several processes, including positive regulation of immune response; positive regulation of leukocyte chemotaxis; and regulation of cytokine production. Acts upstream of or within chemotaxis; homeostasis of number of cells; and immune response. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; immune system; reproductive system; and white fat. Used to study Sjogren's syndrome. Orthologous to human CCR7 (C-C motif chemokine receptor 7).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 211-310 of mouse CCR7 (NP_031745.2).
SequenceSLVSAQVEALITIQVAQMVFGFLVPMLAMSFCYLIIIRTLLQARNFERNKAIKVIIAVVVVFIVFQLPYNGVVLAQTVANFNITNSSCETSKQLNIAYDV
Gene ID
Swiss Prot
SynonymsEBI1; CCR-7; CD197; Ebi1h; Cdw197; Cmkbr7; CC-CKR-7
Calculated MW43kDa
Observed MW43-50kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat spleen
Cellular locationCell membrane ; Multi-pass membrane protein.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - CCR7 Rabbit pAb (A25849)}

    Western blot - CCR7 Rabbit pAb (A25849)

    Western blot analysis of lysates from Rat spleen using CCR7 Rabbit pAb (A25849) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.